They might prove suitable to track accumulated and recent environmental changes as they could represent signs of adaptive evolution. ConclusionĪmino acid polymorphisms within the cytochrome b gene could be assigned to specific geographic locations. Compared to other amino acid variants, L>P263 was remarkably less frequent, and it was predicted using PROVEAN database tool to have non-neutral effects. The variants, L>P263 (Leucine>Proline) and M>T311 (Methionine>Threonine) were specific only to G. andersoni, while the variant V>M283 (Valine>Methionine) was only specific to G. The results demonstrated that amino acid variants L>F173 (Leucine>Phenylalanine), A>M203 (Alanine>Methionine), and I>V221(Isoleucine>Valine) were specific to G. Our results identified a group of amino acid variant polymorphisms that were useful for both species taxonomic and biogeographic assignments. The sequences were obtained from a total of 20 specimens representing four species of genus Gerbillus collected from Siwa Oasis, Dabaa, Wadi El Natron, El Faiyum, and Baltim in Egypt. Amino acid polymorphisms in the mitochondrial cytochrome b (cyt b) gene of four Gerbillus species have been investigated for their geographical distribution and possible functional significance.
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